ARG82837
Chicken IL1 beta ELISA Kit
Chicken IL1 beta ELISA 试剂盒 for ELISA and Chicken
概述
| 产品描述 | ARG82837 Chicken IL1 beta ELISA Kit is an Enzyme Immunoassay kit for the quantification of Chicken IL1 beta in serum, plasma and cell culture supernatants. |
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| 反应物种 | Chk |
| 应用 | ELISA |
| 特异性 | The following recombinant Chicken proteins were tested and exhibited no cross-reactivity or interference: BMP1, BMP2, BMP3, BMP4, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL15, IFN gamma, TGF beta 1, TGF beta 2, TGF beta 3, TLR1, TLR2, TLR3 and TNF alpha. |
| 靶点名称 | IL1 beta |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 31 pg/ml |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | 62 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 7.0% Inter-Assay CV: 10.0% |
| 別名 | Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2 |
应用说明
| 检测时间 | ~ 3 hours |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | IL1B |
|---|---|
| 全名 | interleukin 1, beta |
| 背景介绍 | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008] |
| 生物功能 | Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). [UniProt] |
| 细胞定位 | Cytoplasm, cytosol. Lysosome. Secreted, exosome. Secreted. Note=The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. [UniProt] |
| 翻译后修饰 | Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG82837 Chicken IL1 beta ELISA Kit standard curve image
ARG82837 Chicken IL1 beta ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82837 Chicken IL1 beta ELISA Kit User manual |
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