ARG81357
Dengue virus NS1 ELISA Kit
Dengue virus NS1 ELISA 试剂盒 for ELISA and Dengue virus
Microbiology and Infectious Disease antibody
参考文献7
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81357-001 | antibody-coated microplate | 12 X 8 strips | 4°C |
| ARG81357-002 | Standard | 1 vial | 4°C, store at -20 °C after reconstitution |
| ARG81357-003 | Standard reconstitution buffer | 1.1 mL | 4°C |
| ARG81357-004 | HRP-antibody Conjugate (200X) | 115 μL | 4°C |
| ARG81357-005 | Assay buffer | 12 mL | 4°C |
| ARG81357-006 | Detection antibody diluent buffer | 12 mL | 4°C |
| ARG81357-007 | 10X Wash buffer | 20 mL | 4°C |
| ARG81357-008 | TMB substrate | 12 mL (ready-to-use) | 4°C (Protect from light) |
| ARG81357-009 | STOP solution | 6 mL | 4°C |
| ARG81357-010 | Plate sealer | 1 strips | Room temperature |
概述
| 产品描述 | ARG81357 Dengue virus NS1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Dengue virus NS1 in serum, plasma and cell culture supernatants. |
|---|---|
| 反应物种 | DEN |
| 应用 | ELISA |
| 特异性 | The ELISA kit reacts to DENV type 1-4 NS1 protein and it does not cross-react to ZIKV NS1 protein. |
| 靶点名称 | Dengue virus NS1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | less than 0.5 ng/ml |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | 1.56 - 100 ng/ml |
| 样本量 | 100 µl |
| 別名 | Dengue virus NS1 antibody; Dengue virus nonstructural glycoprotein NS1 antibody |
应用说明
| 检测时间 | ~ 2.5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | DENV_gp1 |
|---|---|
| 全名 | Dengue virus nonstructural protein 1 |
| 背景介绍 | Dengue virus NS1 protein is a nonstructural protein which could be secreted and have been developed as diagnostic biomarker for early detection. There are several forms of NS1 including monomer, dimer, and hexamer during infection. Dimeric NS1 can be anchored to cell membranes with glycosyl-phosphatidylinositol (GPI). Hexameric NS1 can be secreted and detected in patients' blood samples (up to 50 μg/mL) or infected cell supernatants (various from ng/mL to μg/mL depend on serotypes and strains). Studies have shown that NS1 could interfere complement activity and prothrombin activation. In addition, NS1 could elicit antibodies which cross-react with host antigens including coagulation factors and molecules expressed in endothelial cells and platelets through molecular mimic. |
| 生物功能 | Dengue virus (DENV) non-structural protein 1 (NS1) is involved in virus replication and regulation of the innate immune response. Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome. [Uniprot] |
| 产品亮点 | Related products: Dengue Virus antibodies; Dengue Virus ELISA Kits; Dengue Virus Duos / Panels; Related news: New Dengue Virus NS1 antibodies and Antibody Duo Fighting fire with fire: Genetically-engineered mosquitoes as an alternative to fight diseases Tools for studying Dengue Virus Exploring Antiviral Immune Response New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Microbiology and Infectious Disease antibody |
检测图片 (1) Click the Picture to Zoom In
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ARG81357 Dengue virus NS1 ELISA Kit standard curve image
ARG81357 Dengue virus NS1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
文献引用
Urinary dengue NS1 detection on Au-decorated ZnO nanowire platform
ELISA / Cell culture supernatants / Denguevirus
Discrepant Activation Pattern of Inflammation and Pyroptosis Induced in Dermal Fibroblasts in Response to Dengue Virus Serotypes 1 and 2 and Nonstructural Protein 1
ELISA / Dengue virus / Cell culture supernatant
Elevated Interleukin-37 Associated with Dengue Viral Load in Patients with Dengue Fever
ELISA / Dengue virus / serum
DENV NS1 and MMP-9 cooperate to induce vascular leakage by altering endothelial cell adhesion and tight junction.
ELISA / Dengue virus / Cell culture supernatant
Studies on the identification of antiviral lipid molecules against dengue virus using lipidomics.
ELISA / Dengue virus / Culture medium
High levels of serum hyaluronan is an early predictor of dengue warning signs and perturbs vascular integrity
ELISA / Dengue virus
Inhibition of dengue virus infection by 1-stearoyl-2-arachidonoyl-phosphatidylinositol in vitro.
ELISA / Dengue virus
| Title | Download Link |
|---|---|
| ARG81357 Dengue virus NS1 ELISA Kit User's manual |
 Download
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