ARG23757

Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed

Goat anti-Mouse IgG (H+L) 抗体 (FITC), pre-adsorbed for ELISA,ELISPOT,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,Western blot and Mouse

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概述

产品描述 FITC-conjugated Goat Polyclonal antibody recognizes Mouse IgG (H+L)
反应物种 Ms
应用 ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, WB
特异性 Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, and IgG3, and with the light chains of mouse IgM and IgA as demonstrated by ELISA, FLISA, and/or flow cytometry; minimal cross reactivity with human immunoglobulins
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 IgG (H+L)
抗原物种 Mouse
偶联标记 FITC

应用说明

预吸附 Human immunoglobulins and pooled sera.
应用建议
应用 推荐稀释比
ELISAAssay-dependent
ELISPOTAssay-dependent
FACS< 1 µg/10^6 cells
FLISA1:100 - 1:400
ICC/IFAssay-dependent
IHC-FrAssay-dependent
WBAssay-dependent
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS and 0.1% Sodium azide.
抗菌剂 0.1% Sodium azide
浓度 1 mg/ml
存放说明 Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

检测图片 (4) Click the Picture to Zoom In

  • ARG23757 Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed ICC/IF image

    Immunofluorescence: Activated Human neutrophils of AAV patients were stained with ARG23757 Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed and DAPI.

  • ARG23757 Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed FACS image

    Flow Cytometry: Human peripheral blood lymphocytes were stained with Mouse anti-Human CD3 followed by ARG23757 Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed.

  • ARG23757 Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed IHC-P image

    Immunohistochemistry: Paraffin-embedded Human kidney section of AAV patients were stained with ARG23757 Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed and DAPI.

  • ARG23757 Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed ICC/IF image

    Immunofluorescence: Human epithelial carcinoma cell line HEp-2 was stained with Mouse anti-Human CD44 (right) followed by ARG23757 Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed and DAPI.

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Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed

Application:IF/ICC

Sample:Rabbit skeletal muscle

Fixation Buffer:4% PFA

Fixation Time:20 min

Fixation Temperature:25 ºC

Antigen Retrieval Buffer:-

Primary Antibody Dilution Factor:1 : 100

Primary Antibody Incubation Time:Overnight

Primary Antibody Incubation Temperature:4 ºC