ARG23487

Goat anti-Mouse IgG antibody (Biotin), pre-adsorbed

Goat anti-Mouse IgG 抗体 (Biotin), pre-adsorbed for ELISA,ELISPOT,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse

publication_link 参考文献1

概述

产品描述 Biotin-conjugated Goat Polyclonal antibody recognizes Mouse IgG
反应物种 Ms
应用 ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, WB
特异性 Reacts with the heavy chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3.
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 IgG
抗原物种 Mouse
抗原 Mouse IgG
目标Ig IgG
偶联标记 Biotin

应用说明

预吸附 Mouse IgM and IgA; Human immunoglobulins and pooled sera.
应用建议
应用 推荐稀释比
ELISA1:5000 - 1:20000
ELISPOTAssay-dependent
FACS< 1 µg/10^6 cells
FLISAAssay-dependent
ICC/IFAssay-dependent
IHC-Fr1:100 - 1:500
IHC-P1:250 - 1:1500
WBAssay-dependent
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS and 0.1% Sodium azide.
抗菌剂 0.1% Sodium azide
浓度 0.5 mg/ml
存放说明 Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

检测图片 (3) Click the Picture to Zoom In

  • ARG23487 Goat anti-Mouse IgG antibody (Biotin) (pre-adsorbed) ICC/IF image

    Immunofluorescence: Human epithelial carcinoma cell line HEp-2 stained with anti-Human CD44 antibody (right), followed by ARG23487 Goat anti-Mouse IgG antibody (Biotin) (pre-adsorbed), Streptavidin (FITC) and DAPI.

  • ARG23487 Goat anti-Mouse IgG antibody (Biotin) (pre-adsorbed) ELISA image

    ELISA: The plate was coated with purified Mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted ARG23487 Goat anti-Mouse IgG antibody (Biotin) (pre-adsorbed) followed by ARG23912 Streptavidin (HRP).

  • ARG23487 Goat anti-Mouse IgG antibody (Biotin) (pre-adsorbed) FACS image

    Flow Cytometry: Human peripheral blood lymphocytes stained with Mouse anti-Human CD3 antibody followed by ARG23487 Goat anti-Mouse IgG antibody (Biotin) (pre-adsorbed) followed by Streptavidin (FITC).

文献引用

Assembly of Interfacial Polyelectrolyte Complexation Fiber Bundles with Mineralization Gradient for Physiologically-inspired Ligament Regeneration

IHC-P / 

Tzu-Wei Wang et al.
,  (2023)

publication_link

 

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