ARG23806

Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed

Goat anti-Mouse IgG2a 抗体 (Biotin), pre-adsorbed for ELISA,ELISPOT,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Purification,Western blot and Mouse

概述

产品描述 Biotin-conjugated Goat Polyclonal antibody recognizes Mouse IgG2a
反应物种 Ms
应用 ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, Puri, WB
特异性 Reacts with the heavy chain of mouse IgG2a as demonstrated by ELISA, FLISA, and/or flow cytometry. Minimal cross reactivity with human immunoglobulins. May react with immunoglobulins from other species.
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 IgG2a
抗原物种 Mouse
偶联标记 Biotin

应用说明

预吸附 Mouse IgG1, IgG2b, IgG3, IgM, and IgA; Human immunoglobulins and pooled sera.
应用建议
应用 推荐稀释比
ELISA1:5000 - 1:20000
ELISPOTAssay-dependent
FACS< 1 µg/10^6 cells
FLISAAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
PuriAssay-dependent
WBAssay-dependent
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS and 0.1% Sodium azide.
抗菌剂 0.1% Sodium azide
浓度 0.5 mg/ml
存放说明 Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

检测图片 (4) Click the Picture to Zoom In

  • ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed ICC/IF image

    Immunofluorescence: TGFB1 stimulated Human dermal fibroblasts were stained with anti-ACTA2 followed by ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed, Cy3 conjugated Streptavidin, and DAPI.

  • ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed FACS image

    Flow Cytometry: Human blood peripheral lymphocytes were stained with Mouse anti-Human CD5 followed by ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed followed by Streptavidin-FITC.

  • ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed IHC image

    Immunohistochemistry: Paraffin-embedded Human colon cancer tissue was stained with ARG22191 anti-MMP9 antibody [SB15c] followed by ARG23806 Goat anti-Mouse IgG2a antibody (Biotin) (pre-adsorbed), ARG23912 Streptavidin (HRP), DAB, and hematoxylin.

  • ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed ELISA image

    ELISA: ELISA plate was coated with purified Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed followed by Streptavidin-HRP.