ARG21894

Goat anti-Mouse IgG2b antibody (HRP), pre-adsorbed

Goat anti-Mouse IgG2b 抗体 (HRP), pre-adsorbed for ELISA,ELISPOT,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse

概述

产品描述 HRP-conjugated Goat Polyclonal antibody recognizes Mouse IgG2b
反应物种 Ms
应用 ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, WB
特异性 Reacts with the heavy chain of mouse IgG2b
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 IgG2b
抗原物种 Mouse
偶联标记 HRP

应用说明

预吸附 Mouse IgG1, IgG2a, IgG3, IgM, and IgA; Human immunoglobulins and pooled sera.
应用建议
应用 推荐稀释比
ELISA1:4000 - 1:8000
ELISPOTAssay-dependent
FACSAssay-dependent
FLISAAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 50% PBS (pH 7.4) and 50% Glycerol
稳定剂 50% Glycerol
存放说明 Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

检测图片 (7) Click the Picture to Zoom In

  • ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed) ICC image

    Immunocytochemistry: Human pancreatic carcinoma cell line MIA PaCa-2 stained with anti-Histone H3 acetyl (Lys4) antibody followed by ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed), DAB and mounted with Fluoromount-G.

  • ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed) WB image

    Western blot: Jurkat whole cell lysate untreated (left) or TSA-treated (400 nM, 12 hours) (right). The blots were stained with anti-Histone H3 acetyl (Lys4) antibody. Secondary antibody: ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed).

  • ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed) ELISA image

    ELISA: The plate was coated with purified Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed).

  • ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed) ICC image

    Immunocytochemistry: Human pancreatic carcinoma cell line MIA PaCa-2 stained with anti-CD44 antibody and anti-Histone H3 acetyl (Lys4) antibody, followed by ARG23804 Goat anti-Mouse IgG2a antibody (AP) (pre-adsorbed) and ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed), Red AP, DAB and mounted with Fluoromount-G.

  • ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed) ICC image

    Immunocytochemistry: Human pancreatic carcinoma cell line MIA PaCa-2 stained with anti-Histone H3 phospho (Thr3) antibody (right), followed by ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed), DAB and mounted with Fluoromount-G.

  • ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed) ICC image

    Immunocytochemistry: Human pancreatic carcinoma cell line MIA PaCa-2 stained with ARG21506 anti-MMP1 antibody [SB12e] followed by ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed), DAB and mounted with Fluoromount-G.

  • ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed) ICC image

    Immunocytochemistry: Human pancreatic carcinoma cell line MIA PaCa-2 stained with ARG22307 anti-Cytokeratin 8 antibody [SB37b] and ARG21506 anti-MMP1 antibody [SB12e], followed by ARG23804 Goat anti-Mouse IgG2a antibody (AP) (pre-adsorbed), ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed), Red AP, DAB and mounted with Fluoromount-G.