ARG82579
Human BDNF ELISA Kit
Human BDNF ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82579-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82579-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82579-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82579-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82579-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82579-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82579-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82579-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82579-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82579-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82579-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82579 Human BDNF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human BDNF in serum, plasma (EDTA, heparin, citrate), cell culture supernatants and cell lysates. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | BDNF |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin, citrate), cell culture supernatants and cell lysates. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.4% Inter-Assay CV: 6.4% |
| 別名 | Brain-derived neurotrophic factor; Abrineurin; BULN2; ANON2; BDNF |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BDNF |
| 全名 | brain-derived neurotrophic factor |
| 背景介绍 | The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jan 2009] |
| 生物功能 | During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. [UniProt] |
| 细胞定位 | Secreted. Note: A proportion of BDNF is secreted as immature precursor (proBDNF). [UniProt] |
| 产品亮点 | Related products: BDNF antibodies; BDNF ELISA Kits; BDNF recombinant proteins; Related news: The role of HDGF in tumor angiogenesis New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | The propeptide is N-glycosylated and glycosulfated. Converted into mature BDNF by plasmin (PLG). [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG82579 Human BDNF ELISA Kit standard curve image
ARG82579 Human BDNF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82579 Human BDNF ELISA kit User manual |
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