ARG82649
Human C5 / Complement 5 ELISA Kit
Human C5 / Complement 5 ELISA 试剂盒 for ELISA and Human
概述
| 产品描述 | ARG82649 Human C5 / Complement 5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human C5 / Complement 5 in serum, plasma, cell culture supernatants, saliva, milk and CSF. |
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| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | Cross-Reactivity: Monkey: 75% Mouse, Rat, Pig, Dog, Bovine and Rabbit: None No significant cross-reactivity observed with complement C1, C2, C3, C4, C6, C7, C8, C9, factor B, factor D, factor H, factor I and factor P. |
| 靶点名称 | C5 / Complement 5 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 0.084 ng/ml |
| 样品类型 | Serum, plasma, cell culture supernatants, saliva, milk and CSF. |
| 标准范围 | 0.156 - 10 ng/ml |
| 样本量 | 50 µl |
| 精确 | Intra-Assay CV: 4.8% Inter-Assay CV: 9.2% |
| 別名 | ECLZB; CPAMD4; Complement C5; C5D; C5b; C5a; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 |
应用说明
| 检测时间 | ~ 4 hours |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | C5 |
| 全名 | complement component 5 |
| 背景介绍 | This gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the C5 alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mutations in this gene cause complement component 5 deficiency, a disease characterized by recurrent bacterial infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015] |
| 生物功能 | Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed:8182049). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 产品亮点 | Related products: Complement component antibodies; Complement component ELISA Kits; Complement component Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1) Click the Picture to Zoom In
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ARG82649 Human C5 / Complement 5 ELISA Kit standard curve image
ARG82649 Human C5 / Complement 5 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82649 Human C5 / Complement 5 ELISA kit user manual |
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