ARG81497
Human CD258 / LIGHT ELISA Kit
Human CD258 / LIGHT ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81497-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81497-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81497-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81497-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81497-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81497-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81497-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81497-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81497-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81497-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81497-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81497 Human CD258 / LIGHT ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD258 / LIGHT in serum, plasma (heparin, EDTA), saliva and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CD258 / LIGHT |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA), saliva and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.3% Inter-Assay CV: 6.0% |
| 別名 | CD258; Herpes virus entry mediator ligand; HVEML; HVEM-L; Herpesvirus entry mediator ligand; LTg; LIGHT; CD antigen CD258; TR2; Tumor necrosis factor ligand superfamily member 14 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O43557 Human Tumor necrosis factor ligand superfamily member 14 |
|---|---|
| 基因名称 | TNFSF14 |
| 全名 | tumor necrosis factor (ligand) superfamily, member 14 |
| 背景介绍 | The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus. [UniProt] |
| 产品亮点 | Related products: CD258 antibodies; CD258 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | N-glycosylated. The soluble form of isoform 1 derives from the membrane form by proteolytic processing. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81497 Human CD258 / LIGHT ELISA Kit standard curve image
ARG81497 Human CD258 / LIGHT ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81497 Human CD258 / LIGHT ELISA Kit User manual |
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