ARG83029
Human CD274 / PD-L1 ELISA Kit
Human CD274 / PD-L1 ELISA 试剂盒 for ELISA and Human
参考文献1
概述
| 产品描述 | ARG83029 Human CD274 / PD-L1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD274 / PD-L1 in serum, plasma (heparin), ascites, urine and cell culture supernatants. |
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| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | Not cross-reacts with: Human IL2, IL4, IL6, IL10,TNF alpha, TNF beta,PD1. Mouse IL2, IL4, IL5, IL6, IL8, IL10, IL12, PD1,PDL1. Rat IL2, PD1,PDL1. |
| 靶点名称 | CD274 / PD-L1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| Protein Full name | Programmed cell death 1 ligand 1 |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (heparin), ascites, urine and cell culture supernatants. |
| 标准范围 | 156.3 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| 別名 | CD274, CD274 Molecule, PDL1, B7H, PDL1, Programmed Cell Death 1 Ligand 1, CD274 Antigen , B7 Homolog 1, PDCD1LG1, B7 H1, B7 H, PDCD1 Ligand 1, PDCD1L1, HPD-L1, Programmed Death Ligand 1 |
应用说明
| 检测时间 | ~ 4 hours |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD274 |
| 全名 | CD274 Molecule |
| 背景介绍 | This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| 生物功能 | The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy.[UniProt] |
| 细胞定位 | Cell membrane, Early endosome membrane, Recycling endosome membrane. [UniProt] |
| 产品亮点 | Related products: PD-L1 antibodies; PD-L1 ELISA Kits; Related news: Detecting exosomal PD-L1 secreted by cancer cells The best solution for PD-1/PD-L1 research Examining CTL/NK-mediated cytotoxicity by ELISA New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Ubiquitinated; STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation. [Uniprot] |
检测图片 (1) Click the Picture to Zoom In
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ARG83029 Human CD274 / PD L1 ELISA Kit standard curve image
ARG83029 Human CD274 / PD L1 ELISA Kit results of standard run with optical density reading at 450 nm
文献引用
The TOX-RAGE axis mediates inflammatory activation and lung injury in severe pulmonary infectious diseases
ELISA / Human / Plasma
| Title | Download Link |
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| ARG83029 Human CD274 / PD-L1 ELISA Kit User manual |
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