ARG81919
Human CD47 ELISA Kit
Human CD47 ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81919-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81919-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81919-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81919-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81919-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81919-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81919-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81919-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81919-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81919-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81919-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81919 Human CD47 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD47 in serum and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CD47 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 62.5 pg/ml |
| 样品类型 | Serum and cell culture supernatants. |
| 标准范围 | 125 - 8000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.9%; Inter-Assay CV: 6.4% |
| 別名 | Leukocyte surface antigen CD47; CD antigen CD47; Antigenic surface determinant protein OA3; MER6; OA3; Protein MER6; IAP; Integrin-associated protein |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD47 |
| 全名 | CD47 molecule |
| 背景介绍 | This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010] |
| 生物功能 | Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. [UniProt] |
| 产品亮点 | Related products: CD47 antibodies; CD47 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1) Click the Picture to Zoom In
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ARG81919 Human CD47 ELISA Kit standard curve image
ARG81919 Human CD47 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81919 Human CD47 ELISA Kit User's manual |
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