ARG81478

Human COMP ELISA Kit

Human COMP ELISA 试剂盒 for ELISA and Human

内含物

货号 内含物名称 包装 温度
ARG81478-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81478-002 Standard 2 X 10 ng/vial 4°C
ARG81478-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81478-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81478-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81478-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81478-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81478-008 25X Wash buffer 20 ml 4°C
ARG81478-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81478-010 STOP solution 10 ml (Ready to use) 4°C
ARG81478-011 Plate sealer 4 strips Room temperature

概述

产品描述 ARG81478 Human COMP ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human COMP in serum, plasma (heparin, EDTA) and cell culture supernatants.
反应物种 Hu
应用 ELISA
特异性 There is no detectable cross-reactivity with other relevant proteins.
靶点名称 COMP
偶联标记 HRP
偶联标记说明 Substrate: TMB and read at 450 nm.
灵敏度 78 pg/ml
样品类型 Serum, plasma (heparin, EDTA) and cell culture supernatants.
标准范围 156 - 10000 pg/ml
样本量 100 µl
精确 Intra-Assay CV: 5.4%
Inter-Assay CV: 5.8%
別名 MED; TSP5; Thrombospondin-5; COMP; THBS5; EDM1; PSACH; EPD1; Cartilage oligomeric matrix protein

应用说明

检测时间 ~ 5 hours

属性

形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 1311 Human COMP

Swiss-port # P49747 Human Cartilage oligomeric matrix protein

基因名称 COMP
全名 cartilage oligomeric matrix protein
背景介绍 The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). [provided by RefSeq, Jul 2008]
生物功能 May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity). [UniProt]
产品亮点 Related products:
COMP antibodies; COMP ELISA Kits; COMP Duos / Panels;
Related news:
Detecting MMPs and their non-ECM substrates
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData

检测图片 (1) Click the Picture to Zoom In

  • ARG81478 Human COMP ELISA Kit standard curve image

    ARG81478 Human COMP ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81478 Human COMP ELISA Kit User's manual download icon Download