ARG80121
Human CXCL12 / SDF1 alpha ELISA Kit
Human CXCL12 / SDF1 alpha ELISA 试剂盒 for ELISA and Human
Cell Biology and Cellular Response kit; Immune System kit; Microbiology and Infectious Disease kit
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG80121-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80121-002 | Standard (Lyophilized) | 3 X 4 ng/vial | 4°C |
| ARG80121-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80121-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80121-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80121-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80121-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80121-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80121-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80121-010 | STOP solution | 12ml | 4°C |
| ARG80121-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG80121 Human CXCL12 / SDF1 alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CXCL12 / SDF1 alpha in serum, plasma and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | No significant cross-reactivity or interference with Human beta-ECGF, GRO alpha, GRO beta, GRO gamma, MCP-1, MIP-1 alpha, MIP-1 beta, PTN, RANTES；mouse MIP-1 alpha, MIP-1 beta. The cross-reactivity tested with 50 ng/ml of Human SDF-1 beta is <10% and rat SDF-1 beta is <1%. |
| 靶点名称 | CXCL12 / SDF1 alpha |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 62.5 pg/ml |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | 125 - 8000 pg/ml |
| 样本量 | 100 µl |
| 精确 | CV: less than10% |
| 別名 | TPAR1; SDF1; C-X-C motif chemokine 12; Pre-B cell growth-stimulating factor; TLSF; PBSF; SDF-1; Intercrine reduced in hepatomas; IRH; hSDF-1; 3-72; SCYB12; hIRH; 3-67; Stromal cell-derived factor 1 |
应用说明
| 检测时间 | 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CXCL12 |
| 全名 | chemokine (C-X-C motif) ligand 12 |
| 背景介绍 | Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. [provide by uniprot] |
| 产品亮点 | Related products: CXCL12 antibodies; CXCL12 ELISA Kits; CXCL12 recombinant proteins; Related news: LKB1 deficiency in T cells promotes gut tumors New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Cell Biology and Cellular Response kit; Immune System kit; Microbiology and Infectious Disease kit |
| 翻译后修饰 | Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans. |
检测图片 (1) Click the Picture to Zoom In
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ARG80121 Human CXCL12 / SDF1 ELISA Kit standard curve image
ARG80121 Human CXCL12 / SDF1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80121 Human CXCL12 / SDF1 alpha ELISA Kit User manual |
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