ARG80185
Human CXCL2 / MIP2 ELISA Kit
Human CXCL2 / MIP2 ELISA 试剂盒 for ELISA and Human
Immune System kit
参考文献2
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG80185-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80185-002 | Standard (Lyophilized) | 3 X 1 ng/vial | 4°C |
| ARG80185-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80185-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80185-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80185-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80185-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80185-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80185-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80185-010 | STOP solution | 12ml | 4°C |
| ARG80185-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG80185 Human CXCL2 / MIP2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CXCL2 / MIP2 in Serum, Plasma, Cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | No significant cross-reactivity or interference with Human CXCL1, CXCL3, CXCL4, IL-2,3,4,5,6,7,9,10,12, IFN gamma and TNF alpha. |
| 靶点名称 | CXCL2 / MIP2 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 0.8 pg/ml |
| 样品类型 | Serum, Plasma, Cell culture supernatants |
| 标准范围 | 1.56 - 100 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: less than10%; Inter-Assay CV: less than10% |
| 別名 | Gro-beta; SCYB2; HSF; CINC-2a; GROb; MGSA-b; SB-251353; MIP2A; MIP2; Hematopoietic synergistic factor; 5-73; C-X-C motif chemokine 2; MIP-2a; GRO2; Macrophage inflammatory protein 2-alpha; GRO-beta-T; Growth-regulated protein beta; MIP2-alpha |
应用说明
| 检测时间 | 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CXCL2 |
| 全名 | chemokine (C-X-C motif) ligand 2 |
| 背景介绍 | Chemokine (C-X-C motif) ligand 2 (CXCL2) is a small cytokine belonging to the CXC chemokine family that is also called macrophage inflammatory protein 2-alpha (MIP2-alpha), Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2). CXCL2 is 90% identical in amino acid sequence as a related chemokine, CXCL1. This chemokine is secreted by monocytes and macrophages and is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. The gene for CXCL2 is located on human chromosome 4 in a cluster of other CXC chemokines. CXCL2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2. |
| 产品亮点 | Related products: GRO beta antibodies; GRO beta ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Immune System kit |
| 翻译后修饰 | The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells. |
检测图片 (1) Click the Picture to Zoom In
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ARG80185 Human CXCL2 / MIP2ELISA Kit standard curve image
ARG80185 Human CXCL2 / MIP2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
文献引用
Tumor cells educate mesenchymal stromal cells to release chemoprotective and immunomodulatory factors.
ELISA / Human / Cell culture supernatant
Mesenchymal stromal cells release CXCL1/2/8 and induce chemoresistance and macrophage polarization.
ELISA / Human
| Title | Download Link |
|---|---|
| ARG80185 Human GRO beta / CXCL2 ELISA Kit User's manual |
 Download
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