ARG81523
Human CYR61 ELISA Kit
Human CYR61 ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81523-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81523-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81523-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81523-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81523-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81523-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81523-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81523-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81523-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81523-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81523-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81523 Human CYR61 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CYR61 in serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CYR61 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.0% Inter-Assay CV: 7.0% |
| 別名 | Protein GIG1; CCN1; IGFBP-10; Insulin-like growth factor-binding protein 10; IGFBP10; GIG1; Cysteine-rich angiogenic inducer 61; Protein CYR61; CCN family member 1; IGF-binding protein 10; IBP-10 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CYR61 |
| 全名 | cysteine-rich, angiogenic inducer, 61 |
| 背景介绍 | The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011] |
| 生物功能 | Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. [UniProt] |
| 产品亮点 | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1) Click the Picture to Zoom In
-
ARG81523 Human CYR61 ELISA Kit standard curve image
ARG81523 Human CYR61 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81523 Human CYR61 ELISA Kit User manual |
 Download
|
