ARG82648
Human Complement Factor B ELISA Kit
Human Complement Factor B ELISA 试剂盒 for ELISA and Human
概述
| 产品描述 | ARG82648 Human Complement Factor B ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Complement Factor B in serum, plasma, cell culture supernatants, saliva, milk, cell lysate and CSF. |
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| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | Cross-Reactivity: Monkey: <5% Mouse, Rat, Pig, Dog, Bovine and Rabbit: None No significant cross-reactivity observed with complement factor D, factor H, factor I, factor P, C1, C2, C3, C4, C5, C6, C7, C8 and C9. 10% FBS in culture media will not affect the assay. |
| 靶点名称 | Complement Factor B |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 1.1 ng/ml |
| 样品类型 | Serum, plasma, cell culture supernatants, saliva, milk, cell lysate and CSF. |
| 标准范围 | 2.188 - 140 ng/ml |
| 样本量 | 50 µl |
| 精确 | Intra-Assay CV: 5.1% Inter-Assay CV: 9.9% |
| 別名 | Properdin factor B; GBG; BF; PBF2; CFAB; BFD; Complement factor B; CFBD; Glycine-rich beta glycoprotein; FB; ARMD14; C3/C5 convertase; EC 3.4.21.47; FBI12; AHUS4; H2-Bf |
应用说明
| 检测时间 | ~ 4 hours |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
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| 基因名称 | CFB |
| 全名 | complement factor B |
| 背景介绍 | This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2. [provided by RefSeq, Jul 2008] |
| 生物功能 | Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 产品亮点 | Related products: Complement component antibodies; Complement component ELISA Kits; Complement component Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1) Click the Picture to Zoom In
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ARG82648 Human Complement Factor B ELISA Kit standard curve image
ARG82648 Human Complement Factor B ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82648 Human Complement Factor B ELISA kit User manual |
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