ARG82647
Human Complement Factor I ELISA Kit
Human Complement Factor I ELISA 试剂盒 for ELISA and Human
概述
| 产品描述 | ARG82647 Human Complement Factor I ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Complement Factor I in serum, plasma, cell culture supernatants, saliva and milk. |
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| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | Cross-Reactivity: Pig: 75% Rat: 5% Mouse, Monkey, Bovine, Dog and Rabbit: None No significant cross-reactivity observed with complement factor B, factor D, factor H, factor P, C1, C2, C3, C4, C5, C6, C7, C8 and C9. |
| 靶点名称 | Complement Factor I |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 0.28 µg/ml |
| 样品类型 | Serum, plasma, cell culture supernatants, saliva and milk. |
| 标准范围 | 0.375 - 24 µg/ml |
| 样本量 | 25 µl |
| 精确 | Intra-Assay CV: 5.4% Inter-Assay CV: 9.8% |
| 別名 | Complement factor I; KAF; C3B/C4B inactivator; C3b-INA; ARMD13; EC 3.4.21.45; FI; AHUS3; C3BINA; IF |
应用说明
| 检测时间 | ~ 3 hours |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
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| 基因名称 | CFI |
| 全名 | complement factor I |
| 背景介绍 | This gene encodes a serine proteinase that is essential for regulating the complement cascade. The encoded preproprotein is cleaved to produce both heavy and light chains, which are linked by disulfide bonds to form a heterodimeric glycoprotein. This heterodimer can cleave and inactivate the complement components C4b and C3b, and it prevents the assembly of the C3 and C5 convertase enzymes. Defects in this gene cause complement factor I deficiency, an autosomal recessive disease associated with a susceptibility to pyogenic infections. Mutations in this gene have been associated with a predisposition to atypical hemolytic uremic syndrome, a disease characterized by acute renal failure, microangiopathic hemolytic anemia and thrombocytopenia. Primary glomerulonephritis with immune deposits and age-related macular degeneration are other conditions associated with mutations of this gene. [provided by RefSeq, Dec 2015] |
| 生物功能 | Trypsin-like serine protease that plays an essential role in regulating the immune response by controlling all complement pathways. Inhibits these pathways by cleaving three peptide bonds in the alpha-chain of C3b and two bonds in the alpha-chain of C4b thereby inactivating these proteins (PubMed:7360115, PubMed:17320177). Essential cofactors for these reactions include factor H and C4BP in the fluid phase and membrane cofactor protein/CD46 and CR1 on cell surfaces (PubMed:2141838, PubMed:9605165, PubMed:12055245). The presence of these cofactors on healthy cells allows degradation of deposited C3b by CFI in order to prevent undesired complement activation, while in apoptotic cells or microbes, the absence of such cofactors leads to C3b-mediated complement activation and subsequent opsonization (PubMed:28671664). [UniProt] |
| 细胞定位 | Secreted, extracellular space. [UniProt] |
| 产品亮点 | Related products: Complement component antibodies; Complement component ELISA Kits; Complement component Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1) Click the Picture to Zoom In
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ARG82647 Human Complement Factor I ELISA Kit standard curve image
ARG82647 Human Complement Factor I ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82647 Human Complement Factor I ELISA kit User manual |
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