ARG81680
Human E Cadherin ELISA Kit
Human E Cadherin ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81680-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81680-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81680-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81680-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81680-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81680-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81680-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81680-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81680-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81680-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81680-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81680 Human E Cadherin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human E Cadherin in serum, plasma (heparin), saliva, urine and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | E Cadherin |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (heparin), saliva, urine and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.7%; Inter-Assay CV: 6.1% |
| 別名 | Uvomorulin; Arc-1; Cadherin-1; E-cadherin; CDHE; CD antigen CD324; ECAD; CAM 120/80; LCAM; Epithelial cadherin; UVO; CD324 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CDH1 |
| 全名 | cadherin 1, type 1 |
| 背景介绍 | E Cadherin is a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015] |
| 生物功能 | Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria. [UniProt] |
| 产品亮点 | Related products: Cadherin antibodies; Cadherin ELISA Kits; Cadherin Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions. N-glycosylation at Asn-637 is essential for expression, folding and trafficking. Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81680 Human E Cadherin ELISA Kit standard curve image
ARG81680 Human E Cadherin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81680 Human E Cadherin ELISA Kit User manual |
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