ARG70283
Human IGFBP1 recombinant protein (His-tagged, C-ter)
Human IGFBP1 recombinant protein (His-tagged, C-ter) for Binding Activity,SDS-PAGE and Human
概述
| 产品描述 | HEK293 expressed, His-tagged (C-ter) Human IGFBP1 recombinant protein. |
|---|---|
| 反应物种 | Hu |
| 应用 | Binding, SDS-PAGE |
| 靶点名称 | IGFBP1 |
| 物种 | Human |
| A.A. 序列 | Ala26 - Asn259 of Human IGFBP1 (NP_000587.1) with 6X His tag at the C - terminus. |
| 表达系统 | HEK293 |
| 別名 | IBP-1; IBP1; PP12; IGF-BP25; Insulin-like growth factor-binding protein 1; hIGFBP-1; IGFBP-1; Placental protein 12; AFBP; IGF-binding protein 1 |
应用说明
| 应用说明 | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IGFBP-1 at 5µg/ml (100 µl/well) can bind Recombinant Human IGF1 with a linear range of 44-176 ng/ml. |
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属性
| 形式 | Powder |
|---|---|
| 纯化说明 | 0.22 µm filter sterilized. Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 97% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | IGFBP1 |
|---|---|
| 全名 | insulin-like growth factor binding protein 1 |
| 背景介绍 | This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP N-terminal domain and a thyroglobulin type-I domain. The encoded protein, mainly expressed in the liver, circulates in the plasma and binds both insulin-like growth factors (IGFs) I and II, prolonging their half-lives and altering their interaction with cell surface receptors. This protein is important in cell migration and metabolism. Low levels of this protein may be associated with impaired glucose tolerance, vascular disease and hypertension in human patients. [provided by RefSeq, Aug 2017] |
| 生物功能 | IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 预测分子量 | 28 kDa |
| 翻译后修饰 | Phosphorylated; probably by casein kinase II. Phosphorylation alters the affinity of the protein for IGFs. In amniotic fluid, the unmodified protein is the most abundant form, while mono-, bi-, tri- and tetraphosphorylated forms are present in decreasing amounts. The phosphorylation state may influence the propensity to proteolysis. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG70283 Human IGFBP1 recombinant protein (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70283 Human IGFBP1 recombinant protein (His-tagged, C-ter).
