ARG70069
Human IL25 / IL17E recombinant protein (Active) (His-tagged, C-ter)
Human IL25 / IL17E recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
概述
| 产品描述 | E. coli expressed, His-tagged (C-ter) Active Human IL25 / IL17E recombinant protein |
|---|---|
| 应用 | SDS-PAGE |
| 靶点名称 | IL25 / IL17E |
| 物种 | Human |
| A.A. 序列 | Tyr33 - Gly177 |
| 表达系统 | E. coli |
| 生物活性 | Active |
| 活性说明 | Determined by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is < 5 ng/mL. Determined by its ability to induce CXCL1 secretion in HT29 cells. The ED50 for this effect is < 1 ng/mL. |
| 別名 | IL17E; Interleukin-17E; IL-25; Interleukin-25; IL-17E |
属性
| 形式 | Powder |
|---|---|
| 纯化说明 | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 98% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 8.0) |
| 复溶 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | IL25 |
|---|---|
| 全名 | interleukin 25 |
| 背景介绍 | The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010] |
| 生物功能 | Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG70069 Human IL25 / IL17E recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70069 Human IL25 / IL17E recombinant protein (Active) (His-tagged, C-ter).
