ARG82953
Human IL36 beta ELISA kit
Human IL36 beta ELISA 试剂盒 for ELISA and Human
概述
| 产品描述 | ARG82953 Human IL36 beta ELISA kit is an Enzyme Immunoassay kit for the quantification of Human IL36 beta in serum, plasma and cell culture supernatants. |
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| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | IL36 beta |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 7.8 pg/ml |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 μl |
| 精确 | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| 別名 | FIL1; IL1-ETA; FILI-(ETA); IL-1F8; Interleukin-1 homolog 2; IL1H2; IL-1H2; FIL1-(ETA); Interleukin-1 eta; IL-1 eta; IL1F8; FIL1 eta; Interleukin-36 beta; Interleukin-1 family member 8; FIL1H |
应用说明
| 检测时间 | ~ 3.5 hours |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 4°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | IL36B |
| 全名 | interleukin 36, beta |
| 背景介绍 | The protein encoded by this gene is a member of the interleukin 1 cytokine family. Protein structure modeling indicated that this cytokine may contain a 12-stranded beta-trefoil structure that is conserved between IL1A (IL-A alpha) and IL1B (IL-1 beta). This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Stimulates production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. Induces expression of a number of antimicrobial peptides including beta-defensins 4 and 103 as well as a number of matrix metalloproteases. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1, and the production of proinflammatory cytokines such as TNF-alpha, IL-8 and IL-6. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 产品亮点 | Related products: IL36 beta antibodies; IL36 beta ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | N-terminal truncation leads to a dramatic enhancement of its activity (>1000-fold). [UniProt] |
| Title | Download Link |
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| ARG82953 Human IL36 beta ELISA kit User manual |
 Download
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