ARG70272
Human IL6 recombinant protein (Active) (His-tagged, C-ter)
Human IL6 recombinant protein (Active) (His-tagged, C-ter) for Binding Activity,Functional study,SDS-PAGE and Human
概述
| 产品描述 | HEK293 expressed, His-tagged (C-ter) Active Human IL6 recombinant protein. |
|---|---|
| 反应物种 | Hu |
| 应用 | Binding, FuncSt, SDS-PAGE |
| 靶点名称 | IL6 |
| 物种 | Human |
| A.A. 序列 | Val30 - Met212 of Human IL6 (NP_000591.1) with 6X His tag at the C - terminus. |
| 表达系统 | HEK293 |
| 生物活性 | Active |
| 活性说明 | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.12-0.48 pg/ml. |
| 別名 | B-cell stimulatory factor 2; CDF; HSF; BSF-2; Interferon beta-2; IL-6; IFNB2; CTL differentiation factor; Interleukin-6; HGF; Hybridoma growth factor; BSF2; IFN-beta-2 |
应用说明
| 应用说明 | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Human IL6R at 1 ug/ml (100 µl/well) can bind Human IL-6 with a linear range of 2-15 ng/ml. |
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属性
| 形式 | Powder |
|---|---|
| 纯化说明 | 0.22 µm filter sterilized. Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 90% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | IL6 |
|---|---|
| 全名 | interleukin 6 |
| 背景介绍 | This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015] |
| 生物功能 | Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 预测分子量 | 24 kDa |
| 翻译后修饰 | N- and O-glycosylated. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG70272 Human IL6 recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70272 Human IL6 recombinant protein (Active) (His-tagged, C-ter).
