ARG70340
Human K-Ras recombinant protein (His-tagged, N-ter)
Human K-Ras recombinant protein (His-tagged, N-ter) for SDS-PAGE and Human
概述
| 产品描述 | E. coli expressed, His-tagged (N-ter) Human K-Ras recombinant protein. |
|---|---|
| 反应物种 | Hu |
| 应用 | SDS-PAGE |
| 靶点名称 | K-Ras |
| 物种 | Human |
| A.A. 序列 | Thr2 - Cys186 of Human K - Ras (NP_203524.1) with 6X His tag at the N - terminus. |
| 表达系统 | E. coli |
| 別名 | NS3; KRAS1; KRAS2; RALD; K-RAS4A; GTPase KRas; c-Ki-ras; KI-RAS; RASK2; K-RAS2B; K-RAS2A; K-RAS4B; c-K-ras; C-K-RAS; NS; CFC2; Ki-Ras; K-Ras 2 |
属性
| 形式 | Powder |
|---|---|
| 纯化说明 | 0.22 µm filter sterilized. Endotoxin level is <1.0 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 85% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | KRAS |
|---|---|
| 全名 | Kirsten rat sarcoma viral oncogene homolog |
| 背景介绍 | This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008] |
| 生物功能 | Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (PubMed:24623306). [UniProt] |
| 细胞定位 | Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytosol. [UniProt] |
| 预测分子量 | 22 kDa |
| 翻译后修饰 | Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG70340 Human K-Ras recombinant protein (His-tagged, N-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70340 Human K-Ras recombinant protein (His-tagged, N-ter).
