ARG81952
Human LDL receptor ELISA Kit
Human LDL receptor ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81952-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81952-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81952-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81952-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81952-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81952-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81952-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81952-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81952-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81952-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81952-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81952 Human LDL receptor ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human LDL receptor in serum, plasma (heparin), urine and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | LDL Receptor |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 31.25 pg/ml |
| 样品类型 | Serum, plasma (heparin), urine and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.1%; Inter-Assay CV: 6.6% |
| 別名 | FH; LDLCQ2; Low-density lipoprotein receptor; LDL receptor; FHC |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | LDLR |
| 全名 | low density lipoprotein receptor |
| 背景介绍 | The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010] |
| 生物功能 | Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. [UniProt] |
| 产品亮点 | Related products: LDL Receptor antibodies; LDL Receptor ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | N- and O-glycosylated. Ubiquitinated by MYLIP leading to degradation. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81952 Human LDL receptor ELISA Kit standard curve image
ARG81952 Human LDL receptor ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81952 Human LDL receptor ELISA Kit User's manual |
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