ARG82580
Human Leptin ELISA Kit
Human Leptin ELISA 试剂盒 for ELISA and Human
Cell Biology and Cellular Response kit; Developmental Biology kit; Metabolism kit; Neuroscience kit; Signaling Transduction kit
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82580-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82580-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82580-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82580-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82580-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82580-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82580-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82580-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82580-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82580-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82580-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82580 Human Leptin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Leptin in Serum, Plasma (EDTA. Heparin) , Cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | Leptin |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 31.25 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.5% Inter-Assay CV: 7.8% |
| 別名 | Leptin; Obese protein; OB; LEPD; Obesity factor; OBS |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | LEP |
| 全名 | leptin |
| 背景介绍 | This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008] |
| 生物功能 | May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. [UniProt] |
| 产品亮点 | Related products: Leptin antibodies; Leptin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Cell Biology and Cellular Response kit; Developmental Biology kit; Metabolism kit; Neuroscience kit; Signaling Transduction kit |
检测图片 (1) Click the Picture to Zoom In
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ARG82580 Human Leptin ELISA Kit standard curve image
ARG82580 Human Leptin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82580 Human Leptin ELISA kit User manual |
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