ARG82043
Human Leptin Receptor ELISA Kit
Human Leptin Receptor ELISA 试剂盒 for ELISA and Human
概述
| 产品描述 | Human Leptin Receptor ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Leptin Receptor in serum, plasma and cell culture supernatants. |
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| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | Leptin Receptor |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 156 pg/ml |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | 312 - 20000 pg/ml |
| 样本量 | 100 µl |
| 別名 | LEPRD; CD antigen CD295; CD295; Leptin receptor; LEP-R; HuB219; OB-R; OBR; OB receptor |
应用说明
| 检测时间 | 4.5 hours |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
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| 基因名称 | LEPR |
| 全名 | leptin receptor |
| 背景介绍 | The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010] |
| 生物功能 | Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway (By similarity). [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. Basolateral cell membrane. Isoform E: Secreted. [UniProt] |
| 产品亮点 | Related products: Leptin Receptor antibodies; Leptin Receptor ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation,for interaction with SOCS3 and SOCS3 mediated inhibition of leptin signaling. Phosphorylation on Tyr-1141 is required for STAT3 binding/activation. Phosphorylation of Tyr-1079 has a more accessory role. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG82043 Human Leptin Receptor ELISA Kit standard curve image
ARG82043 Human Leptin Receptor ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82043 Human Leptin Receptor ELISA Kit User manual |
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