ARG80145

Human MMP1 (Total) ELISA kit

Human MMP1 (Total) ELISA 试剂盒 for ELISA and Human

Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit
publication_link 参考文献1

内含物

货号 内含物名称 包装 温度
ARG80145-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80145-002 Standard (Lyophilized) 3 X 2 ng/vial 4°C
ARG80145-003 Standard diluent buffer 20 ml 4°C
ARG80145-004 Antibody conjugate concentrate 400 µl 4°C
ARG80145-005 Antibody diluent buffer 16 ml 4°C
ARG80145-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80145-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80145-008 20X Wash buffer 50 ml 4°C
ARG80145-009 TMB substrate 12ml 4°C (Protect from light)
ARG80145-010 STOP solution 12ml 4°C
ARG80145-011 Plate sealer 4 strips Room temperature

概述

产品描述 ARG80145 Human MMP1 (Total) ELISA kit is an Enzyme Immunoassay kit for the quantification of Human MMP1 (Total) in Serum, Plasma, Cell culture supernatants.
反应物种 Hu
应用 ELISA
特异性 No significant cross-reactivity or interference with Human MMP-2,3,7,8,9,10,12,13,16, TIMP-1,2,3,4
靶点名称 MMP1
偶联标记 HRP
偶联标记说明 Substrate: TMB and read at 450 nm
灵敏度 30 pg/ml
样品类型 Serum, Plasma, Cell culture supernatants
标准范围 62.5 - 4000 pg/ml
样本量 100 µl
精确 CV: less than10%
別名 MMP-1; CLG; Fibroblast collagenase; Matrix metalloproteinase-1; CLGN; EC 3.4.24.7; Interstitial collagenase

应用说明

检测时间 4 hours

属性

形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 4312 Human MMP1

Swiss-port # P03956 Human Interstitial collagenase

基因名称 MMP1
全名 matrix metallopeptidase 1
背景介绍 The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterizes the structure of the MMPs. In addition, fibronectin-like repeats, a hinge region, and a C-terminal hemopexin-like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane-type MMP subfamilies. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer's, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis.
产品亮点 Related products:
MMP1 antibodies; MMP1 ELISA Kits;
Related news:
Detecting MMPs and their non-ECM substrates
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
研究领域 Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit
翻译后修饰 Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
Tyrosine phosphorylated in platelets by PKDCC/VLK.

检测图片 (1) Click the Picture to Zoom In

  • ARG80145 Human MMP1 (Total) ELISA kit standard curve image

    ARG80145 Human MMP1 (Total) ELISA kit results of a typical standard run with optical density reading at 450 nm.

文献引用

Adipose-derived stem cells enriched with therapeutic mRNA TGF-β3 and IL-10 synergistically promote scar-less wound healing in preclinical models

ELISA / Human / Culture supernatant

Wei Wang et al.
Bioengineering & Translational Medicine,  (2023)

publication_link

 

hr_line
Title Download Link
ARG80145 Human MMP1 (Total) ELISA kit User's manual download icon Download