ARG82633
Human MMP14 / MT1-MMP Assay Kit
Human MMP14 / MT1-MMP Assay 试剂盒 for Functional study and Human
概述
| 产品描述 | ARG82633 Human MMP14 / MT1-MMP Assay Kit is a detection kit for the quantification of Human MMP14 in cell and tissue lysates. |
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| 反应物种 | Hu |
| 应用 | FuncSt |
| 特异性 | Measures endogenous active MMP14 (naturally occurring). |
| 靶点名称 | MMP14 / MT1-MMP |
| 偶联标记说明 | Read at 405 nm. |
| 灵敏度 | 500 pg/ml for 2 hours incubation 100 pg/ml for 5 hours incubation |
| 样品类型 | Cell and tissue lysates |
| 标准范围 | 100 - 16000 pg/ml |
| 样本量 | 100 µl |
| 別名 | MT1MMP; MT-MMP 1; Membrane-type matrix metalloproteinase 1; MT1-MMP; Membrane-type-1 matrix metalloproteinase; MT-MMP; EC 3.4.24.80; MMP-X1; MMP-14; Matrix metalloproteinase-14; WNCHRS; MTMMP1 |
应用说明
| 检测时间 | Overnight |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MMP14 |
| 全名 | matrix metallopeptidase 14 (membrane-inserted) |
| 背景介绍 | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008] |
| 生物功能 | Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. [UniProt] |
| 细胞定位 | Membrane; Single-pass type I membrane protein. Melanosome. Cytoplasm. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm. [UniProt] |
| 翻译后修饰 | The precursor is cleaved by a furin endopeptidase. Tyrosine phosphorylated by PKDCC/VLK. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG82633 Human MMP14 / MT1-MMP Assay Kit typical data demonstration image
ARG82633 Human MMP14 / MT1-MMP Assay Kit results of a typical data with optical density reading at 405 nm (after 2 hours incubation).
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ARG82633 Human MMP14 / MT1-MMP Assay Kit typical data demonstration image
ARG82633 Human MMP14 / MT1-MMP Assay Kit results of a typical data with optical density reading at 405 nm (after 5 hours incubation).
| Title | Download Link |
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| ARG82633 Human MMP14 Assay kit User manual |
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