ARG82631
Human MMP9 Assay Kit
Human MMP9 Assay 试剂盒 for Functional study and Human
概述
| 产品描述 | ARG82631 Human MMP9 Assay Kit is a detection kit for the quantification of Human MMP9 in serum, plasma, cell culture supernatants, urine, synovial fluid and tissue homogenates. |
|---|---|
| 反应物种 | Hu |
| 应用 | FuncSt |
| 特异性 | Measures endogenous active MMP9 (naturally occurring) or total active MMP9 (following activation with APMA). |
| 靶点名称 | MMP9 |
| 偶联标记说明 | Read at 405 nm. |
| 灵敏度 | 500 pg/ml for 1 hour incubation 10 pg/ml for 4 hours incubation |
| 检测范围 | 10 - 16000 pg/ml |
| 样品类型 | Serum, plasma, cell culture supernatants, urine, synovial fluid and tissue homogenates. |
| 样本量 | 100 µl |
| 別名 | Matrix metalloproteinase-9; 92 kDa gelatinase; MMP-9; Gelatinase B; GELB; CLG4B; MANDP2; EC 3.4.24.35; 92 kDa type IV collagenase |
应用说明
| 检测时间 | Overnight |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MMP9 |
| 全名 | matrix metallopeptidase 9 |
| 背景介绍 | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008] |
| 生物功能 | May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. [UniProt] |
| 细胞定位 | Secreted, extracellular space, extracellular matrix. [UniProt] |
| 翻译后修饰 | Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. N- and O-glycosylated. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG82631 Human MMP9 Assay Kit typical data demonstration image
ARG82631 Human MMP9 Assay Kit results of a typical data with optical density reading at 405 nm (after 1 hour incubation).
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ARG82631 Human MMP9 Assay Kit typical data demonstration image
ARG82631 Human MMP9 Assay Kit results of a typical data with optical density reading at 405 nm (after 4 hours incubation).
| Title | Download Link |
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| ARG82631 Human MMP9 Assay kit User manual |
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