ARG80129
Human MMP9 ELISA Kit
Human MMP9 ELISA 试剂盒 for ELISA and Human
Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit
参考文献6
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG80129-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80129-002 | Standard (Lyophilized) | 3 X 2 ng/vial | 4°C |
| ARG80129-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80129-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80129-005 | Antibody diluent buffer | 20 ml | 4°C |
| ARG80129-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80129-007 | HRP-Streptavidin diluent buffer | 20 ml | 4°C |
| ARG80129-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80129-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80129-010 | STOP solution | 12ml | 4°C |
| ARG80129-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG80129 Human MMP9 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MMP9 in Serum, Plasma, Cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | No significant cross-reactivity or interference with Human MMP-1,2,3,7,8,10,12,13,14;TIMP-2,3,4 |
| 靶点名称 | MMP9 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, Plasma, Cell culture supernatants |
| 标准范围 | 31.25 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | CV: less than10% |
| 別名 | Matrix metalloproteinase-9; 92 kDa gelatinase; MMP-9; Gelatinase B; GELB; CLG4B; MANDP2; EC 3.4.24.35; 92 kDa type IV collagenase |
应用说明
| 检测时间 | 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MMP9 |
| 全名 | matrix metallopeptidase 9 |
| 背景介绍 | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008] |
| 生物功能 | May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. [UniProt] |
| 产品亮点 | Related products: MMP9 antibodies; MMP9 ELISA Kits; MMP9 Duos / Panels; Related news: Tumor microenvironments are shown to affect progression of several cancer subtypes Rat MMP9 ELISA Kit is available in arigo New MMP7 (total or active) ELISA kits are released Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit |
| 翻译后修饰 | Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. N- and O-glycosylated. |
检测图片 (1) Click the Picture to Zoom In
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ARG80129 Human MMP9 ELISA Kit standard curve image
ARG80129 Human MMP9 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
文献引用
Conditioned medium from amniotic fluid mesenchymal stem cells could modulate Alzheimer's disease-like changes in human neuroblastoma cell line SY-SY5Y in a paracrine manner
ELISA / Human / Cell culture supernatant
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ELISA / Human / Serum
Executive dysfunction in euthymic bipolar disorder patients and its association with plasma biomarkers
ELISA / Human / Plasma
Identification of potential plasma protein biomarkers for bipolar II disorder: a preliminary/exploratory study.
ELISA / Human / Plasma
Tear Proteomics Study of Dry Eye Disease: Which Eye Do You Adopt as the Representative Eye for the Study?
ELISA / Human / Tear fluid
Tear Proteomics Approach to Monitoring Sjögren Syndrome or Dry Eye Disease.
ELISA / Human / Tear fluid
| Title | Download Link |
|---|---|
| ARG80129 Human MMP9 ELISA Kit User's manual |
 Download
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