ARG80142
Human PLGF ELISA Kit
Human PLGF ELISA 试剂盒 for ELISA and Human
Cell Biology and Cellular Response kit
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG80142-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80142-002 | Standard (Lyophilized) | 2 X 3 ng/vial | 4°C |
| ARG80142-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80142-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80142-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80142-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80142-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80142-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80142-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80142-010 | STOP solution | 12ml | 4°C |
| ARG80142-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG80142 Human PLGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PLGF in serum, plasma and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | No significant cross-reactivity or interference with Human EGF, PDGF-AA, PDGF-BB, VEGF121, VEGF165 and mouse VEGF165. |
| 靶点名称 | PLGF |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 15 pg/ml |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | 31.25 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | CV: less than10% |
| 別名 | PGFL; PlGF; PlGF-2; SHGC-10760; Placenta growth factor; D12S1900; PLGF |
应用说明
| 检测时间 | 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PGF |
| 全名 | placental growth factor |
| 背景介绍 | This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011] |
| 生物功能 | Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. [UniProt] |
| 细胞定位 | Secreted. Note=The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin. [UniProt] |
| 产品亮点 | Related products: PLGF antibodies; PLGF ELISA Kits; Related news: The role of HDGF in tumor angiogenesis New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Cell Biology and Cellular Response kit |
| 翻译后修饰 | N-glycosylated. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG80142 Human PLGF1 ELISA Kit standard curve image
ARG80142 Human PLGF1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80142 Human PLGF1 ELISA Kit User's manual |
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