ARG81429
Human PROK1 / EG-VEGF ELISA Kit
Human PROK1 / EG-VEGF ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81429-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81429-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81429-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81429-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81429-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81429-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81429-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81429-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81429-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81429-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81429-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81429 Human PROK1 / EG-VEGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PROK1 / EG-VEGF in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | PROK1 / EG-VEGF |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 7.8 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.2% Inter-Assay CV: 7.2% |
| 別名 | PK1; Endocrine-gland-derived vascular endothelial growth factor; Prokineticin-1; PRK1; EG-VEGF; Mambakine; EGVEGF |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PROK1 |
| 全名 | prokineticin 1 |
| 背景介绍 | The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner. [provided by RefSeq, Sep 2011] |
| 生物功能 | Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis. [UniProt] |
| 产品亮点 | Related products: PROK1 antibodies; PROK1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1) Click the Picture to Zoom In
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ARG81429 Human PROK1 / EG-VEGF ELISA Kit standard curve image
ARG81429 Human PROK1 / EG-VEGF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81429 Human PROK1 / EG-VEGF ELISA Kit User's manual |
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