ARG81597
Human RSPO1 ELISA Kit
Human RSPO1 ELISA 试剂盒 for ELISA and Human
参考文献1
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81597-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81597-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81597-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81597-004 | Antibody conjugate concentrate (100X) | 1 vial (120 µl) | 4°C |
| ARG81597-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81597-006 | HRP-Streptavidin concentrate (100X) | 1 vial (120 µl) | 4°C |
| ARG81597-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81597-008 | 25X Wash buffer | 30 ml | 4°C |
| ARG81597-009 | TMB substrate | 12 ml (Ready to use) | 4°C (Protect from light) |
| ARG81597-010 | STOP solution | 12 ml (Ready to use) | 4°C |
| ARG81597-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81597 Human RSPO1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human RSPO1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | RSPO1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.1% Inter-Assay CV: 5.9% |
| 別名 | hRspo1; Roof plate-specific spondin-1; R-spondin-1; RSPO; CRISTIN3 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RSPO1 |
| 全名 | R-spondin 1 |
| 背景介绍 | This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
| 生物功能 | Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. [UniProt] |
| 产品亮点 | Related products: RSPO1 antibodies; RSPO1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1) Click the Picture to Zoom In
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ARG81597 Human RSPO1 ELISA Kit standard curve image
ARG81597 Human RSPO1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
文献引用
Human artificial chromosome carrying R-spondin1 and IL-22 expression cassettes in rejuvenated MSCs enhances therapeutic efficacy in ulcerative colitis model mice
ELISA / Mouse / Plasma, cell culture supernatant
| Title | Download Link |
|---|---|
| ARG81597 Human RSPO1 ELISA Kit User's manual |
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