ARG70415
Human SCF recombinant protein (Active) (His-tagged, C-ter)
Human SCF recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
概述
| 产品描述 | E. coli expressed, His-tagged (C-ter) Active Human SCF recombinant protein |
|---|---|
| 应用 | SDS-PAGE |
| 靶点名称 | SCF |
| 物种 | Human |
| A.A. 序列 | Glu26 - Ala189 |
| 表达系统 | E. coli |
| Protein Full name | Kit ligand |
| 生物活性 | Active |
| 活性说明 | Determined by its ability to induce TF-1 cells proliferation. The ED50 for this effect is < 5 ng/mL. The specific activity of recombinant human SCF is > 5 x 10^5 IU/mg. |
| 別名 | KITLG; KIT Ligand; SCF; Mast Cell Growth Factor; Stem Cell Factor; DFNA69; Kitl; KL-1 FPH2; SLF 2; MGF; SF; Familial Progressive Hyperpigmentation 2; Steel Factor; C-Kit Ligand; Kit Ligand; SHEP7; CUA; FPHH; WS2F |
属性
| 形式 | Powder |
|---|---|
| 纯化说明 | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 98% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | KITLG |
|---|---|
| 全名 | KIT Ligand |
| 背景介绍 | This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. |
| 生物功能 | Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. |
| 细胞定位 | Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Secreted |
| 翻译后修饰 | Disulfide bond; Glycoprotein |
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ARG70415 Human SCF recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70415 Human SCF recombinant protein (Active) (His-tagged, C-ter)
