ARG81526

Human SPARC ELISA Kit

Human SPARC ELISA 试剂盒 for ELISA and Human

内含物

货号 内含物名称 包装 温度
ARG81526-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81526-002 Standard 2 X 50 ng/vial 4°C
ARG81526-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81526-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81526-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81526-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81526-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81526-008 25X Wash buffer 20 ml 4°C
ARG81526-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81526-010 STOP solution 10 ml (Ready to use) 4°C
ARG81526-011 Plate sealer 4 strips Room temperature

概述

产品描述 ARG81526 Human SPARC ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human SPARC in serum, human milk and cell culture supernatants.
反应物种 Hu
应用 ELISA
特异性 There is no detectable cross-reactivity with other relevant proteins.
靶点名称 SPARC
偶联标记 HRP
偶联标记说明 Substrate: TMB and read at 450 nm.
灵敏度 0.39 ng/ml
样品类型 Serum, human milk and cell culture supernatants.
标准范围 0.78 - 50 ng/ml
样本量 100 µl
精确 Intra-Assay CV: 6.5%
Inter-Assay CV: 7.5%
別名 ON; BM-40; Basement-membrane protein 40; SPARC; Osteonectin; Secreted protein acidic and rich in cysteine

应用说明

检测时间 ~ 5 hours

属性

形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 6678 Human SPARC

Swiss-port # P09486 Human SPARC

基因名称 SPARC
全名 secreted protein, acidic, cysteine-rich (osteonectin)
背景介绍 This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]
生物功能 Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. [UniProt]
产品亮点 Related products:
SPARC antibodies; SPARC ELISA Kits;
Related news:
Detecting MMPs and their non-ECM substrates
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData

检测图片 (1) Click the Picture to Zoom In

  • ARG81526 Human SPARC ELISA Kit standard curve image

    ARG81526 Human SPARC ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81526 Human SPARC ELISA Kit User's manual download icon Download