ARG83634
Human Siglec 5 + Siglec 14 ELISA kit
Human Siglec 5 + Siglec 14 ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG83634-01 | Antibody Coated Microplate | 8 X 12 strips | 4°C |
| ARG83634-02 | Standard (Lyophilized) | 3 vials | 4°C |
| ARG83634-03 | Standard diluent buffer | 20 ml | 4°C |
| ARG83634-04 | Antibody conjugate concentrate | 1 vials | 4°C |
| ARG83634-05 | Antibody diluent buffer | 16 ml | 4°C |
| ARG83634-06 | HRP-Streptavidin concentrate | 1 vials | 4°C (protect from light) |
| ARG83634-07 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG83634-08 | 20X Wash buffer | 50 ml | 4°C |
| ARG83634-09 | TMB substrate | 12 ml | 4°C (protect from light) |
| ARG83634-10 | STOP solution | 12 ml | 4°C |
| ARG83634-11 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG83634 Human Siglec 5 + Siglec 14 ELISA kit for the quantification of Human Siglec 5 + Siglec 14 in serum, plasma (heparin), ascites, urine and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | Not cross-reacts with: Siglec 3/Fc Chimera ; Siglec 7/Fc Chimera ; Siglec 9/Fc Chimera |
| 靶点名称 | Siglec 5 + Siglec 14 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 23.5 pg/ml |
| 样品类型 | Serum, plasma (heparin), ascites, urine and cell culture supernatants. |
| 标准范围 | 46.9 - 3000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| 別名 | OB-BP2 ; SIGLEC-5 ; CD33L2 ; CD170 ; Sialic Acid-Binding Ig-Like Lectin 5 ; Obesity-Binding Protein 2 ; OB-Binding Protein 2 ; CD33 Antigen-Like 2 ; OBBP2 ; Sialic Ac ; d-Binding Immunoglobulin-Like Lectin 5 ; Sialic Acid Binding Ig-Like Lectin 5 ; CD170 Antigen ; Siglec-5 / Sialic Acid-Binding Ig-Like Lectin 14 ; Siglec-14 |
应用说明
| 检测时间 | ~ 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | SIGLEC5 + SIGLEC14 |
|---|---|
| 全名 | Sialic Acid Binding Ig Like Lectin 5 + Sialic Acid Binding Ig Like Lectin 14 |
| 背景介绍 | Siglec 5 encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion. [provided by RefSeq, Feb 2012] Siglec 14 predicted to enable sialic acid binding activity. Predicted to be involved in cell adhesion. Predicted to be located in ficolin-1-rich granule membrane and tertiary granule membrane. Predicted to be active in plasma membrane. [provided by Alliance of Genome Resources, Dec 2024] |
| 生物功能 | Siglec 5:Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. [UniProt]] Siglec 14:Putative adhesion molecule. Sialic acid-binding paired receptor which may activate associated receptors. [UniProt] |
| 细胞定位 | Membrane [UniProt] |
| 产品亮点 | Related products: Siglec antibodies; Siglec ELISA Kits; Siglec Duos / Panels New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Disulfide bond, Glycoprotein [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG83634 Human Siglec 5 + Siglec 14 ELISA kit standard curve image
ARG83634 Human Siglec 5 + Siglec 14 ELISA kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG83634 Human Siglec 5 + Siglec 14 ELISA kit User's manual |
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