ARG70107
Human TGF beta 2 recombinant protein (Active) (His-tagged, C-ter)
Human TGF beta 2 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
概述
| 产品描述 | E. coli expressed, His-tagged (C-ter) Active Human TGF beta 2 recombinant protein |
|---|---|
| 应用 | SDS-PAGE |
| 靶点名称 | TGF beta 2 |
| 物种 | Human |
| A.A. 序列 | Ala303 - Ser414 |
| 表达系统 | E. coli |
| 生物活性 | Active |
| 活性说明 | Determined by its ability to inhibit IL-4-induce proliferation in HT-2 cells. The ED50 for this effect is < 0.2 ng/mL. The specific activity of recombinant human TGF beta 2 is > 5 x 10^6 IU/mg. |
| 別名 | TGF-beta2; Cetermin; LDS4; Polyergin; Glioblastoma-derived T-cell suppressor factor; G-TSF; Transforming growth factor beta-2; LAP; TGF-beta-2; BSC-1 cell growth inhibitor |
属性
| 形式 | Powder |
|---|---|
| 纯化说明 | Endotoxin level is < 0.01 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 98% (by SDS-PAGE) |
| 缓冲液 | 20 mM Sodium citrate and 0.2 M NaCl (pH 3.5). |
| 复溶 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not <100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | TGFB2 |
|---|---|
| 全名 | transforming growth factor, beta 2 |
| 背景介绍 | This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types by transducing their signal through combinations of transmembrane type I and type II receptors (TGFBR1 and TGFBR2) and their downstream effectors, the SMAD proteins. Disruption of the TGFB/SMAD pathway has been implicated in a variety of human cancers. The encoded protein is secreted and has suppressive effects of interleukin-2 dependent T-cell growth. Translocation t(1;7)(q41;p21) between this gene and HDAC9 is associated with Peters' anomaly, a congenital defect of the anterior chamber of the eye. The knockout mice lacking this gene show perinatal mortality and a wide range of developmental, including cardiac, defects. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010] |
| 生物功能 | TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 翻译后修饰 | The precursor is cleaved into mature TGF-beta-2 and LAP, which remains non-covalently linked to mature TGF-beta-2 rendering it inactive. [UniProt] |
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ARG70107 Human TGF beta 2 recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70107 Human TGF beta 2 recombinant protein (Active) (His-tagged, C-ter).
