ARG81882
Human TGF beta Receptor 2 ELISA Kit
Human TGF beta Receptor 2 ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81882-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81882-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81882-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81882-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81882-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81882-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81882-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81882-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81882-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81882-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81882-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81882 Human TGF beta Receptor 2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TGF beta Receptor 2 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | TGF beta Receptor 2 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 7.8 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.3%; Inter-Assay CV: 6.0% |
| 別名 | FAA3; AAT3; TbetaR-II; TGF-beta receptor type-2; LDS2B; MFS2; TGF-beta type II receptor; EC 2.7.11.30; TAAD2; TGFR-2; LDS2; Transforming growth factor-beta receptor type II; TGFbeta-RII; TGF-beta receptor type II; RIIC; LDS1B |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TGFBR2 |
| 全名 | transforming growth factor, beta receptor II (70/80kDa) |
| 背景介绍 | This gene encodes a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized. [provided by RefSeq, Jul 2008] |
| 生物功能 | Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. [UniProt] |
| 产品亮点 | Related products: TGF beta Receptor antibodies; TGF beta Receptor ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Phosphorylated on a Ser/Thr residue in the cytoplasmic domain. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
-
ARG81882 Human TGF beta Receptor 2 ELISA Kit standard curve image
ARG81882 Human TGF beta Receptor 2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81882 Human TGF beta Receptor 2 ELISA Kit User's manual |
 Download
|
