ARG70284
Human TGFBR2 / TGF beta Receptor II recombinant protein (Fc-His-tagged, C-ter)
Human TGFBR2 / TGF beta Receptor II recombinant protein (Fc-His-tagged, C-ter) for Binding Activity,SDS-PAGE and Human
概述
| 产品描述 | HEK293 expressed, Fc-His-tagged (C-ter) Human TGFBR2 / TGF beta Receptor II recombinant protein. |
|---|---|
| 反应物种 | Hu |
| 应用 | Binding, SDS-PAGE |
| 靶点名称 | TGFBR2 / TGF beta Receptor II |
| 物种 | Human |
| A.A. 序列 | Thr23 - Asp159 of Human TGFBR2 / TGF beta Receptor II (NP_003233.4) with an Fc - 6X His tag at the C - terminus. |
| 表达系统 | HEK293 |
| 別名 | FAA3; AAT3; TbetaR-II; TGF-beta receptor type-2; LDS2B; MFS2; TGF-beta type II receptor; EC 2.7.11.30; TAAD2; TGFR-2; LDS2; Transforming growth factor-beta receptor type II; TGFbeta-RII; TGF-beta receptor type II; RIIC; LDS1B |
应用说明
| 应用说明 | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized recombinant human TGFB1 at 2 ug/ml (100 µl/well) can bind recombinant human TGFBR2 with a linear range of 1-5 ng/ml. |
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属性
| 形式 | Powder |
|---|---|
| 纯化说明 | 0.22 µm filter sterilized. Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | TGFBR2 |
|---|---|
| 全名 | transforming growth factor, beta receptor II (70/80kDa) |
| 生物功能 | Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. Membrane raft. [UniProt] |
| 预测分子量 | 65 kDa |
| 翻译后修饰 | Phosphorylated on a Ser/Thr residue in the cytoplasmic domain. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG70284 Human TGFBR2 / TGF beta Receptor II recombinant protein (ECD) (Fc-His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70284 Human TGFBR2 / TGF beta Receptor II recombinant protein (ECD) (Fc-His-tagged, C-ter).
