ARG80187
Human TIMP1 ELISA Kit
Human TIMP1 ELISA 试剂盒 for ELISA and Human
Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG80187-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80187-002 | Standard (Lyophilized) | 2 X 1 ng/vial | 4°C |
| ARG80187-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80187-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80187-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80187-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80187-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80187-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80187-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80187-010 | STOP solution | 12ml | 4°C |
| ARG80187-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG80187 Human TIMP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TIMP1 in Serum, Plasma, Cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | No significant cross-reactivity or interference with Human MMP-1, MMP-3, MMP-2, TIMP-2；mouse TIMP-1 |
| 靶点名称 | TIMP1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 16 pg/ml |
| 样品类型 | Serum, Plasma, Cell culture supernatants |
| 标准范围 | 31.25 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | CV: less than10% |
| 別名 | Erythroid-potentiating activity; TIMP; Collagenase inhibitor; Fibroblast collagenase inhibitor; TIMP-1; EPO; CLGI; Tissue inhibitor of metalloproteinases 1; HCI; Metalloproteinase inhibitor 1; EPA |
应用说明
| 检测时间 | 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TIMP1 |
| 全名 | TIMP metallopeptidase inhibitor 1 |
| 背景介绍 | This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq] |
| 产品亮点 | Related products: TIMP1 antibodies; TIMP1 ELISA Kits; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit |
| 翻译后修饰 | The activity of TIMP1 is dependent on the presence of disulfide bonds. N-glycosylated. |
检测图片 (1) Click the Picture to Zoom In
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ARG80187 Human TIMP1 ELISA Kit standard curve image
ARG80187 Human TIMP1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80187 Human TIMP1 ELISA Kit User's manual |
 Download
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