ARG81475
Human TLR2 ELISA Kit
Human TLR2 ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81475-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81475-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81475-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81475-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81475-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81475-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81475-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81475-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81475-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81475-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81475-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81475 Human TLR2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TLR2 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | TLR2 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.2% Inter-Assay CV: 5.9% |
| 別名 | CD antigen CD282; TIL4; CD282; Toll/interleukin-1 receptor-like protein 4; Toll-like receptor 2 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TLR2 |
| 全名 | toll-like receptor 2 |
| 背景介绍 | The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. [UniProt] |
| 产品亮点 | Related products: TLR2 antibodies; TLR2 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81475 Human TLR2 ELISA Kit standard curve image
ARG81475 Human TLR2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81475 Human TLR2 ELISA Kit User's manual |
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