ARG81422
Human TNF Receptor II ELISA Kit
Human TNF Receptor II ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81422-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81422-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81422-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81422-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81422-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81422-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81422-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81422-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81422-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81422-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81422-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81422 Human TNF Receptor II ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TNF Receptor II in serum, plasma (heparin, EDTA, citrate), urine and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | TNFR2 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 3.9 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA, citrate), urine and cell culture supernatants. |
| 标准范围 | 7.8 - 500 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.6% Inter-Assay CV: 6.3% |
| 別名 | TNFR1B; CD antigen CD120b; p75; CD120b; p75TNFR; TNF-R-II; TNFBR; TBPII; TNFR80; TBP-2; TNFR2; Tumor necrosis factor receptor 2; Tumor necrosis factor receptor type II; Etanercept; Tumor necrosis factor receptor superfamily member 1B; TNFR-II; TNF-R75; TNF-R2; TNF-RII; p80 TNF-alpha receptor |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P20333 Human Tumor necrosis factor receptor superfamily member 1B |
|---|---|
| 基因名称 | TNFRSF1B |
| 全名 | tumor necrosis factor receptor superfamily, member 1B |
| 背景介绍 | The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. [provided by RefSeq, Jul 2008] |
| 生物功能 | Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. [UniProt] |
| 产品亮点 | Related products: TNFR antibodies; TNFR ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Phosphorylated; mainly on serine residues and with a very low level on threonine residues. A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81422 Human TNF Receptor II ELISA Kit standard curve image
ARG81422 Human TNF Receptor II ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81422 Human TNF Receptor II ELISA Kit User's manual |
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