ARG81305
Human VEGF165 ELISA Kit
Human VEGF165 ELISA 试剂盒 for ELISA and Human
参考文献1
概述
| 产品描述 | ARG81305 Human VEGF165 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human VEGF165 in serum, plasma and cell culture supernatants. |
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| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | No cross reaction with VEGF-121, VEGF-145, VEGF-B, VEGF-C, VEGF-D, VEGF-R1, Ang-2, GM-CSF, IFN gamma, IL-29. Shows cross reactivity with VEGF-189 isoform. |
| 靶点名称 | VEGF165 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.9 pg/ml |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | 31.25 - 1000 pg/ml |
| 样本量 | 50 µl |
| 別名 | MVCD1; Vascular permeability factor; VEGF-A; VPF; VEGF; Vascular endothelial growth factor A |
应用说明
| 检测时间 | ~ 4 hours |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P15692 Human Vascular endothelial growth factor A |
|---|---|
| 基因名称 | VEGFA |
| 全名 | vascular endothelial growth factor A |
| 背景介绍 | This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008] |
| 生物功能 | Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. [UniProt] |
| 产品亮点 | Related products: VEGF antibodies; VEGF ELISA Kits; VEGF Duos / Panels; VEGF recombinant proteins; Related news: The role of HDGF in tumor angiogenesis New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1) Click the Picture to Zoom In
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ARG81305 Human VEGF165 ELISA Kit standard curve image
ARG81305 Human VEGF165 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
文献引用
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ELISA / Human
| Title | Download Link |
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| ARG81305 Human VEGF165 ELISA Kit User's manual |
 Download
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