ARG82004
Human alpha Defensin 1 ELISA Kit
Human alpha Defensin 1 ELISA 试剂盒 for ELISA and Human
参考文献1
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82004-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82004-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82004-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82004-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82004-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82004-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82004-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82004-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82004-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82004-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82004-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82004 Human alpha Defensin 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human alpha Defensin 1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | Cross Reactivity: There is no detectable cross-reactivity with recombinant human α-Defensin 5, β-Defensin 3, C1qB, MCP-2 and Serpin F2 and the kit might also react to HNP2 and HNP3. |
| 靶点名称 | alpha Defensin 1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 156 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 312 - 20000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.2%; Inter-Assay CV: 5.5% |
| 別名 | DEF1; Neutrophil defensin 1; HNP-1; HNP-2; HP-2; HP1; Defensin, alpha 1; MRS; DEFA2; HP2; HP-1 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DEFA1 |
| 全名 | defensin, alpha 1 |
| 背景介绍 | Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. [provided by RefSeq, Oct 2014] |
| 生物功能 | Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 产品亮点 | Related products: alpha Defensin 1 antibodies; alpha Defensin 1 ELISA Kits; Related news: New MMP7 (total or active) ELISA kits are released New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | ADP-ribosylation drastically reduces cytotoxic and antibacterial activities, and enhances IL8 production. Phosphorylation at Tyr-85 has been found in some cancer cell lines, and interferes with ADP-ribosylation. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG82004 Human alpha Defensin 1 ELISA Kit standard curve image
ARG82004 Human alpha Defensin 1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
文献引用
Serum proteins may facilitate the identification of Kawasaki disease and promote in vitro neutrophil infiltration.
ELISA / Human / Serum
| Title | Download Link |
|---|---|
| ARG82004 Human alpha Defensin 1 ELISA Kit User's manual |
 Download
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