ARG80130
Human sCD40L ELISA Kit
Human sCD40L ELISA 试剂盒 for ELISA and Human
Cell Biology and Cellular Response kit; Developmental Biology kit; Immune System kit
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG80130-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80130-002 | Standard (Lyophilized) | 3 X 2 ng/vial | 4°C |
| ARG80130-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80130-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80130-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80130-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80130-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80130-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80130-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80130-010 | STOP solution | 12ml | 4°C |
| ARG80130-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG80130 Human sCD40L ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human sCD40L in Serum, Plasma, Cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | No significant cross-reactivity or interference with Human ANG, AR, BDNF, CD4, CD40, CNTF, CT-1, CTLA-4, Epo, Fas, CDNF, GITR, OPG, OSM |
| 靶点名称 | sCD40L |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 30 pg/ml |
| 样品类型 | Serum, Plasma, Cell culture supernatants |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | CV: less than10% |
| 別名 | TNFSF5; IMD3; T-cell antigen Gp39; HIGM1; CD40-L; gp39; CD40 ligand; Tumor necrosis factor ligand superfamily member 5; CD40L; CD154; TRAP; CD antigen CD154; hCD40L; IGM; T-BAM; TNF-related activation protein |
应用说明
| 检测时间 | 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD40LG |
| 全名 | CD40 ligand |
| 背景介绍 | The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008] |
| 生物功能 | Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. [UniProt] |
| 产品亮点 | Related products: CD154 antibodies; CD154 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Cell Biology and Cellular Response kit; Developmental Biology kit; Immune System kit |
| 翻译后修饰 | The soluble form derives from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Not O-glycosylated. |
检测图片 (1) Click the Picture to Zoom In
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ARG80130 Human sCD40L ELISA Kit standard curve image
ARG80130 Human sCD40L ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80130 Human sCD40L ELISA Kit User's manual |
 Download
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