ARG80125
Human sCD54 / sICAM1 ELISA Kit
Human sCD54 / sICAM1 ELISA 试剂盒 for ELISA and Human
Cell Biology and Cellular Response kit; Developmental Biology kit; Immune System kit
参考文献3
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG80125-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80125-002 | Standard (Lyophilized) | 3 X 30 ng/vial | 4°C |
| ARG80125-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80125-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80125-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80125-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80125-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80125-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80125-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80125-010 | STOP solution | 12ml | 4°C |
| ARG80125-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG80125 Human sCD54 / sICAM1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human sCD54 / sICAM1 in serum, plasma and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | No significant cross-reactivity or interference with Human sCD31, ICAM-2, sE-Selectin, sL-Selectin, sVCAM-1；mouse VCAM-1 |
| 靶点名称 | sCD54 / sICAM1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 0.24 ng/ml |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | 0.47 - 30 ng/ml |
| 样本量 | 100 µl |
| 精确 | CV: less than10% |
| 別名 | CD54; CD antigen CD54; BB2; P3.58; Intercellular adhesion molecule 1; Major group rhinovirus receptor; ICAM-1 |
应用说明
| 检测时间 | 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ICAM1 |
| 全名 | intercellular adhesion molecule 1 |
| 背景介绍 | This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008] |
| 生物功能 | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity). [UniProt] |
| 产品亮点 | Related products: CD54 antibodies; CD54 ELISA Kits; CD54 Duos / Panels; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Cell Biology and Cellular Response kit; Developmental Biology kit; Immune System kit |
| 翻译后修饰 | Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. |
检测图片 (1) Click the Picture to Zoom In
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ARG80125 Human sCD54 / sICAM1 ELISA Kit standard curve image
ARG80125 Human sCD54 / sICAM1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
文献引用
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| Title | Download Link |
|---|---|
| ARG80125 Human sICAM1 ELISA Kit User's manual |
 Download
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