ARG80183

Human sRANK Ligand / TNFSF11 / TRANCE ELISA Kit

Datasheet

参考文献1

订购信息

包装

价格 (USD)

96 wells

439
250

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

内含物

货号	内含物名称	包装	温度
ARG80183-001	Antibody-coated microplate	8 X 12 strips	4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80183-002	Standard (Lyophilized)	3 X 2 ng/vial	4°C
ARG80183-003	Standard diluent buffer	20 ml	4°C
ARG80183-004	Antibody conjugate concentrate	400 µl	4°C
ARG80183-005	Antibody diluent buffer	16 ml	4°C
ARG80183-006	HRP-Streptavidin concentrate	400 µl	4°C (Protect from light)
ARG80183-007	HRP-Streptavidin diluent buffer	16 ml	4°C
ARG80183-008	20X Wash buffer	50 ml	4°C
ARG80183-009	TMB substrate	12ml	4°C (Protect from light)
ARG80183-010	STOP solution	12ml	4°C
ARG80183-011	Plate sealer	4 strips	Room temperature

概述

产品描述	ARG80183 Human sRANK Ligand / TNFSF11 / TRANCE ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human sRANK Ligand / TNFSF11 / TRANCE in Serum, Plasma, Cell culture supernatants.
反应物种	Hu
应用	ELISA
特异性	No significant cross-reactivity or interference with Human IL-2,3,4,5,6,7,9,10,12, IFN- gamma, TNF- alpha
靶点名称	sRANK Ligand / TNFSF11 / TRANCE
偶联标记	HRP
偶联标记说明	Substrate: TMB and read at 450 nm
灵敏度	8 pg/ml
样品类型	Serum, Plasma, Cell culture supernatants
标准范围	15.6 - 1000 pg/ml
样本量	100 µl
精确	CV: less than10%
別名	TRANCE; Osteoprotegerin ligand; CD254; sOdf; Receptor activator of nuclear factor kappa-B ligand; OPTB2; RANKL; OPGL; Tumor necrosis factor ligand superfamily member 11; hRANKL2; TNF-related activation-induced cytokine; Osteoclast differentiation factor; ODF; CD antigen CD254

应用说明

检测时间	4 hours

属性

形式	96 well
存放说明	Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 8600 Human TNFSF11 Swiss-port # O14788 Human Tumor necrosis factor ligand superfamily member 11
基因名称	TNFSF11
全名	tumor necrosis factor (ligand) superfamily, member 11
背景介绍	This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced tra
产品亮点	Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData
研究领域	Cell Biology and Cellular Response kit; Immune System kit
翻译后修饰	The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.

检测图片 (1) Click the Picture to Zoom In

ARG80183 Human sRANK Ligand / TNFSF11 / TRANCE ELISA Kit standard curve image

ARG80183 Human sRANK Ligand / TNFSF11 / TRANCE ELISA Kit results of a typical standard run with optical density reading at 450 nm.

文献引用

sTNFRII-Fc modification protects human UC-MSCs against apoptosis/autophagy induced by TNF-α and enhances their efficacy in alleviating inflammatory arthritis.

ELISA / Human / Cell culture medium

Yingjie Zhao et al.
Preprint., (2021)