ARG30273

LC3A and LC3B Antibody Duo

Datasheet

参考文献6

订购信息

包装

价格 (USD)

1 pair

478

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

内含物

货号	内含物名称	宿主克隆性	反应	应用	包装
ARG51300	anti-LC3A antibody	Rabbit pAb	Hu, Ms, Rat	ICC/IF, IHC-P, WB	50 μl
ARG55799	anti-LC3B antibody	Rabbit pAb	Hu, Ms, Rat	ICC/IF, IHC-P, WB	50 μl

概述

产品描述	Autophagy consists of a destructive mechanism that disassemble unnecessary or dysfunctional cellular components through a regulated process. Targeted cellular constituents are enclosed by double-membraned vesicle called autophagosome. LC3s (MAP1-LC3A, B and C) are structural proteins of autophagosomal membranes, widely used as biomarkers of autophagy. They are processed from their precursors and bound to PE to form autophagosome before being fused to lysosome and breakdown the enclosed components. In the cytoplasm, there was a minimal co-localization between LC3A and B staining, suggesting that the relevant autophagosomes are formed by only one out of the two LC3 proteins. Koukourakis M. et al. 2015. Plos One 17(10) Related news: Keap1-Nrf2-ARE antibody panel is launched Freeze! Scientists found a way to stop tumor migration Therapeutic strategies against PDAC
靶点名称	LC3A and LC3B
別名	LC3A and LC3B antibody; Microtubule-associated protein 1 light chain 3 alpha and Microtubule-associated protein 1 light chain 3 beta antibody; LC3A antibody; LC3B antibody

属性

存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

全名	Microtubule-associated protein 1 light chain 3 alpha (LC3A) and Microtubule-associated protein 1 light chain 3 beta (LC3B) Antibody Duo
产品亮点	Related products: LC3 antibodies; LC3 Duos / Panels;

检测图片 (9) Click the Picture to Zoom In

ARG51300 anti-LC3A antibody WB image

Western blot: 30 µg of HeLa untreated or treated with CQ (50 µM) and stained with ARG51300 anti-LC3A antibody at 1:1000 dilution.
ARG55799 anti-LC3B antibody WB image (Customer's Feedback)

Western blot: HeLa cells untreated or treated with Chloroquine. 20 µg of cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody and ARG55799 anti-LC3B antibody at 1:1000 dilution.

An anti-GAPDH antibody used as a positive control.
ARG55799 anti-LC3B antibody ICC/IF image

Immunofluorescence: NIH/3T3 cells treated with Chloroquine (50 μM, 37°C for 20 hours). Cells were stained with ARG55799 anti-LC3B antibody at 1:100 dilution.
ARG55799 anti-LC3B antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Rat kidney stained with ARG55799 anti-LC3B antibody at 1:100 dilution.
ARG51300 anti-LC3A antibody ICC/IF image

Immunofluorescence: methanol-fixed HeLa cells stained with anti-LC3A antibody ARG51300.
ARG51300 anti-LC3A antibody WB image

Western blot: 30 µg of HeLa cell lysate stained with ARG51300 anti-LC3A antibody at 1:500 dilution.
ARG51300 anti-LC3A antibody WB image

Western blot: 30 µg of HeLa cells untreated or treated with Chloroquine at 50 µM or 100 µM (18 hr). The blots stained with ARG51300 anti-LC3A antibody at 1:500 dilution.
ARG55799 anti-LC3B antibody WB image

Western blot: 30 µg of HeLa untreated or treated with Chloroquine at 50 µM, 100 µM (18 hr). The bolts stained with ARG55799 anti-LC3B antibody at 1:1000 dilution.
ARG55799 anti-LC3B antibody WB image

Western blot: 30 µg of SW620, Mouse spleen, and Rat spleen lysates stained with ARG55799 anti-LC3B antibody at 1:1000 dilution.