ARG82672
Monkey Perforin ELISA Kit
Monkey Perforin ELISA 试剂盒 for ELISA and Monkey
概述
| 产品描述 | ARG82672 Monkey Perforin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Monkey Perforin in serum, plasma and cell culture supernatants. |
|---|---|
| 反应物种 | Mk |
| 应用 | ELISA |
| 靶点名称 | Perforin |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 20 pg/ml |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | 39 - 2500 pg/ml |
| 样本量 | 100 µl |
| 精确 | CV: less than 10% |
| 別名 | FLH2; P1; PFN1; Cytolysin; HPLH2; PFP; Lymphocyte pore-forming protein; Perforin-1 |
应用说明
| 检测时间 | ~ 3.5 hours |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | PRF1 |
|---|---|
| 全名 | perforin 1 (pore forming protein) |
| 背景介绍 | This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017] |
| 生物功能 | Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. [UniProt] |
| 细胞定位 | Cytoplasmic granule lumen. Secreted. Cell membrane; Multi-pass membrane protein. Endosome lumen. Note=Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes. [UniProt] |
| 产品亮点 | Related products: Perforin antibodies; Perforin ELISA Kits; Related news: Examining CTL/NK-mediated cytotoxicity by ELISA New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | N-glycosylated. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG82672 Monkey Perforin ELISA Kit standard curve image
ARG82672 Monkey Perforin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82672 Monkey Perforin ELISA kit User manual |
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