ARG81660
Mouse CCL20 / MIP3 alpha ELISA Kit
Mouse CCL20 / MIP3 alpha ELISA 试剂盒 for ELISA and Mouse
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81660-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81660-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81660-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81660-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81660-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81660-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81660-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81660-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81660-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81660-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81660-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81660 Mouse CCL20 / MIP3 alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CCL20 / MIP3 alpha in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CCL20 / MIP3 alpha |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 3.9 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 7.8 - 500 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.4%; Inter-Assay CV: 7.4% |
| 別名 | ST38; CC chemokine LARC; Exodus; Macrophage inflammatory protein 3 alpha; 2-70; MIP-3a; Beta-chemokine exodus-1; SCYA20; MIP-3-alpha; LARC; MIP3A; 1-64; Small-inducible cytokine A20; 1-67; CKb4; C-C motif chemokine 20; Liver and activation-regulated chemokine |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CCL20 |
| 全名 | chemokine (C-C motif) ligand 20 |
| 背景介绍 | This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014] |
| 生物功能 | Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. [UniProt] |
| 产品亮点 | Related products: CCL20 antibodies; CCL20 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81660 Mouse CCL20 / MIP3 alpha ELISA Kit standard curve image
ARG81660 Mouse CCL20 / MIP3 alpha ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81660 Mouse CCL20 / MIP3 alpha ELISA Kit user's manual |
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